Mezzo-soprano Marissia Papalexiou studied at the School of Law of the University of Athens and singing at the Athens Conservatoire, from which she graduated with honours. She completed her musical studies in Düsseldorf with the mezzo-soprano Jane Henschel.

As a soloist, she has collaborated with all of the Greek orchestras, performing works from the operatic and oratorio repertoire as well as works by contemporary Greek composers. She works with the Greek National Opera, Athens Festival and Staatstheater Cottbus, where she has variously appeared as Maddalena (Rigoletto), Suzuki (Madama Butterfly), Carmen, Prince Orlofsky (Die Fledermaus), Bersi (Andrea Chénier), Fenena (Nabucco), Sonyetka (Lady Macbeth of Mtsensk), Nicklaus (The Tales of Hoffmann) and Jenny (Mahagonny).