The Rodolfus Choir is made up of singers aged 25 and younger who have been chosen from past and present members of the Eton College Choral Courses for prospective choral scholars. Each year these six-week-long summer courses attract some 350 students between the ages of sixteen and twenty, and of these perhaps a dozen are invited to join the Rodolfus Choir as places become vacant. Many of these singers are choral scholars, some are at music college, and most hope to make a career in music.

Since its foundation in 1984 the choir has toured on the continent, and has appeared at many English festivals including the Three Choirs Festival in Gloucester, the BBC Proms, Ryedale, Three Choirs, Snape Proms and York Early Music Festival. It has also performed throughout the United Kingdom at various other venues. The choir has made three recordings and recorded Songs of Praise. Its recent recordings include music as diverse as Monteverdi, Grier, Tallis and German Romantic motets.